Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation

Senthamizhchelvan, Srinivasan; Pant, G.S.; Rath, G.K.; Julka, P.K.; Nair, O.

doi:10.1016/j.ejmp.2008.05.004

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Volume 25, Issue 2 , Pages 82-87, June 2009

Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation

Srinivasan Senthamizhchelvan
- Department of Nuclear Medicine, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India
- Department of Radiotherapy, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India
- Corresponding author. Department of Radiotherapy, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India.
G.S. Pant
- Department of Nuclear Medicine, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India
G.K. Rath
- Department of Radiotherapy, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India
P.K. Julka
- Department of Radiotherapy, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India
O. Nair
- Department of Radiotherapy, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India

Received 14 October 2007; received in revised form 2 May 2008; accepted 16 May 2008. published online 01 July 2008.

Abstract

Biological dosimetry provides information on the absorbed dose and its distribution in the body for an early assessment of irradiation consequences in exposed individuals. In this study, an effort has been made to see the applicability of biological dosimetry using micronuclei assay for dose estimation in therapeutic irradiation of cancer patients in acute high dose partial body irradiation. Dose estimation in partial body irradiations was done on the basis of Dolphin's contaminated Poisson method, using the in vitro dose response calibration curve. The equivalent whole body dose and the dose to the irradiated part of the body were estimated to be 1.8±0.1Gy and 6.4±0.3Gy, respectively. The estimated percentage of irradiated blood and the fraction of cells exposed were 41.5±1.6% and 10.4±0.8%, respectively. The estimated fraction of irradiated cells was comparable with the actual volume of irradiation.